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rabbit anti zfh 1  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank rabbit anti zfh 1
    Rabbit Anti Zfh 1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+zfh+1/anti-Osteonectin/pm37683644-257-6-40
    Average 93 stars, based on 20 article reviews
    rabbit anti zfh 1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Germline self-renewal requires cyst stem cells, while stat regulates niche adhesion in Drosophila testes
    Article Snippet: .. Primary antibodies were incubated overnight in block, and were goat anti-Vasa (1:400, Santa Cruz sc-26877), mouse anti-Fasciclin III (1:50, Developmental Studies Hybridoma Bank), rabbit anti-Zfh-1 (1:5000, Ruth Lehmann), rabbit anti-Stat (1:1000, Erika Bach), mouse anti-HA (1:600, Covance), rabbit anti-GFP (1:1000, Molecular Probes), rabbit anti-alpha-spectrin (1:600, Dan Kiehart, Duke Univ., N.C.), rabbit anti-Ptb (1:200, David Standiford), mouse anti-lacZ (1:10,000, Promega), rabbit anti-phosphohistone H3 (1:500, Upstate Biotech), rabbit anti-centrosomin (1:1000, Thomas Kaufman, Bloomington, In), mouse anti-γtubulin (1:100, Sigma Clone GTU-88), rat anti-E-Cadherin (1:20, Developmental Studies Hybridoma Bank), mouse anti-phosphoSmad (1:1000, Carl-Henrik Heldin), and guinea pig anti-traffic jam (1:10,000, Dorothea Godt). .. Secondary antibodies conjugated to A488, Cy3, or Cy5 (Molecular Probes and Jackson Immunologicals) were used at 1:400.

    Blocking Assay:

    Article Title: Germline self-renewal requires cyst stem cells, while stat regulates niche adhesion in Drosophila testes
    Article Snippet: .. Primary antibodies were incubated overnight in block, and were goat anti-Vasa (1:400, Santa Cruz sc-26877), mouse anti-Fasciclin III (1:50, Developmental Studies Hybridoma Bank), rabbit anti-Zfh-1 (1:5000, Ruth Lehmann), rabbit anti-Stat (1:1000, Erika Bach), mouse anti-HA (1:600, Covance), rabbit anti-GFP (1:1000, Molecular Probes), rabbit anti-alpha-spectrin (1:600, Dan Kiehart, Duke Univ., N.C.), rabbit anti-Ptb (1:200, David Standiford), mouse anti-lacZ (1:10,000, Promega), rabbit anti-phosphohistone H3 (1:500, Upstate Biotech), rabbit anti-centrosomin (1:1000, Thomas Kaufman, Bloomington, In), mouse anti-γtubulin (1:100, Sigma Clone GTU-88), rat anti-E-Cadherin (1:20, Developmental Studies Hybridoma Bank), mouse anti-phosphoSmad (1:1000, Carl-Henrik Heldin), and guinea pig anti-traffic jam (1:10,000, Dorothea Godt). .. Secondary antibodies conjugated to A488, Cy3, or Cy5 (Molecular Probes and Jackson Immunologicals) were used at 1:400.

    Immunostaining:

    Article Title: The Drosophila actin regulator ENABLED regulates cell shape and orientation during gonad morphogenesis.
    Article Snippet: .. Immunostaining and in situ hybridization Antibody staining was conducted as described previously [11], using the following primary antibodies: mouse anti-Ena (5G2, 1:200; C. Goodman, DSHB), rabbit anti-Vas (1:20,000; Lehmann, R.), chick anti-Vas (1:300; Lehmann, R.), rabbit anti-Zfh-1 (1:4,000; Lehmann, R.), rabbit anti-Serpent (1:500; D. Hoshizaki), rabbit anti-GFP (1:1,000; Clontech), rat anti-DE-cad (DCAD2, 1:50; T. Uemura, DSHB). ..

    In Situ Hybridization:

    Article Title: The Drosophila actin regulator ENABLED regulates cell shape and orientation during gonad morphogenesis.
    Article Snippet: .. Immunostaining and in situ hybridization Antibody staining was conducted as described previously [11], using the following primary antibodies: mouse anti-Ena (5G2, 1:200; C. Goodman, DSHB), rabbit anti-Vas (1:20,000; Lehmann, R.), chick anti-Vas (1:300; Lehmann, R.), rabbit anti-Zfh-1 (1:4,000; Lehmann, R.), rabbit anti-Serpent (1:500; D. Hoshizaki), rabbit anti-GFP (1:1,000; Clontech), rat anti-DE-cad (DCAD2, 1:50; T. Uemura, DSHB). ..

    Article Title: Tbx20-related genes, mid and H15, are required for tinman expression, proper patterning, and normal differentiation of cardioblasts in Drosophila.
    Article Snippet: Tbx20-related T-box genes have been implicated in the regulation of heart development in several vertebrate species.. In the present report, we demonstrate that a pair of genes representing Drosophila orthologs of Tbx20, midline (mid) and H15, have important functions during the development of the Drosophila equivalent of the heart, i.e. the dorsal vessel.. We show that mid is among the earliest known genes that are specifically expressed in all cardioblasts during early embryogenesis, and H15 expression is subsequently activated in the same cells.

    Staining:

    Article Title: The Drosophila actin regulator ENABLED regulates cell shape and orientation during gonad morphogenesis.
    Article Snippet: .. Immunostaining and in situ hybridization Antibody staining was conducted as described previously [11], using the following primary antibodies: mouse anti-Ena (5G2, 1:200; C. Goodman, DSHB), rabbit anti-Vas (1:20,000; Lehmann, R.), chick anti-Vas (1:300; Lehmann, R.), rabbit anti-Zfh-1 (1:4,000; Lehmann, R.), rabbit anti-Serpent (1:500; D. Hoshizaki), rabbit anti-GFP (1:1,000; Clontech), rat anti-DE-cad (DCAD2, 1:50; T. Uemura, DSHB). ..

    Generated:

    Article Title: dRTEL1 is essential for the maintenance of Drosophila male germline stem cells
    Article Snippet: .. The following primary antibodies were used in this study: guinea pig anti-Vasa (1:10,000, a gift from Toshie Kai); rabbit anti-Zfh-1 (1:20,000, [ ]); chicken anti-GFP (1:5,000, Abcam); rabbit anti-α-Spectrin (1:3,000, [ ]); mouse anti-α-Spectrin (3A9, 1:200, monoclonal from Developmental Studies Hybridoma Bank (DSHB)); mouse anti-FasIII (7G10, 1:100, monoclonal from DSHB); rabbit anti-pMad (1:500, Cell Signaling); mouse anti-Flag (1:2,000, Sigma); rabbit-anti-Cleaved Caspase-3 (1:1000, ab208003, Abcam); mouse anti-γ-H2Av (UNC93-5.2.1, 1:2,000, monoclonal from DSHB); mouse anti-p53 (Dmp53 H3, 1:2,000, monoclonal from DSHB); guinea pig anti-Stat92E (1:1000, generated in our lab). ..



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